BLAST Search Results

BLASTX 2.2.11 [Jun-05-2005]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Database: /dbase/web_blast/db/Efflux_Pump.fasta 
           24,506 sequences; 14,206,867 total letters



Query= gi|126373828|gb|CP000522.1| Acinetobacter baumannii ATCC
17978 plasmid pAB1, complete sequence
         (13,408 letters)

Distribution of 13 Blast Hits on the Query Sequence



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

gi|148524819|dbj|BAF63417.1| resolvase [Escherichia coli]              50   6e-05 
gi|27262957|emb|CAD59442.1| putative LysR family transcriptional...    47   4e-04 
gi|7801208|emb|CAB91105.1| TtgS protein [Pseudomonas putida]           46   9e-04 
gi|13926035|gb|AAK49514.1|AF321092_1 TtgS [Pseudomonas putida]         39   0.11  
gi|149824947|gb|EDM84159.1| acriflavin resistance protein [Limno...    37   0.53  
gi|149926129|ref|ZP_01914391.1| acriflavin resistance protein [L...    37   0.53  
gi|126098068|gb|ABN74896.1| probable macrolide-specific efflux p...    35   2.0   
gi|126209276|ref|YP_001054501.1| probable macrolide-specific eff...    35   2.0   
gi|149822944|gb|EDM82189.1| RND family efflux transporter [Limno...    35   2.0   
gi|149928359|ref|ZP_01916600.1| RND family efflux transporter [L...    35   2.0   
gi|108736213|gb|ABG00342.1| RepB [Lactococcus lactis]                  34   3.4   
gi|91710870|gb|ABE50798.1| acriflavin resistance protein [Methyl...    33   4.5   
gi|91776883|ref|YP_546639.1| acriflavin resistance protein [Meth...    33   4.5   

>gi|148524819|dbj|BAF63417.1| resolvase [Escherichia coli]
          Length = 185

 Score = 49.7 bits (117), Expect = 6e-05
 Identities = 44/192 (22%), Positives = 79/192 (41%), Gaps = 1/192 (0%)
 Frame = -3

Query: 7661 YLRASTKDQDAKRALQILQDLNQNLNLGETIVYVENYSGTKLDRPELNKLLSEANQGDTL 7482
            Y R ST  Q     ++ L+D     N     ++ +  SG+  DR  L+ L  +  +GD +
Sbjct: 6    YARVSTSQQSLDLQVRALKDAGVKANR----IFTDKASGSSTDREGLDLLRMKVEEGDVI 61

Query: 7481 LVESIDRLSRLTQRDFQELKR-RIQEKGLRLVVADLPTTYQMIQASDSITHSILELINNM 7305
            LV+ +DRL R T    Q +K    Q   +R +   + T   M Q               M
Sbjct: 62   LVKKLDRLGRDTADMIQLIKEFDAQGVAVRFIDDGISTDGDMGQ---------------M 106

Query: 7304 LIDLLATMARLDNEKRIERIKQGLARSGYKPTGKKANEAKHKRIKELLVAGNMTKEEIAK 7125
            ++ +L+ +A+ +  + +ER  +G   +  K           + +   L        EIA 
Sbjct: 107  VVTILSAVAQAERRRILERTNEGRQEAKLKGIKFGRRRTVDRNVVLTLHQKGTGATEIAH 166

Query: 7124 AVNCGVATVYRV 7089
             ++   +TVY++
Sbjct: 167  QLSIARSTVYKI 178



>gi|27262957|emb|CAD59442.1| putative LysR family transcriptional
            regulator [Pseudomonas putida]
          Length = 304

 Score = 47.0 bits (110), Expect = 4e-04
 Identities = 39/161 (24%), Positives = 69/161 (42%), Gaps = 1/161 (0%)
 Frame = -3

Query: 3452 LFNIDMNLTHRQLTMFRAIMLHGNLGRAADACASSQPTLSRELGRLEQLLGFNLFDRVRG 3273
            L  +D+NL    L +F  +M   ++ RAA+     QP +S  L RL  L    LF R   
Sbjct: 6    LRRVDLNL----LIVFETLMHERSVTRAAEKLFLGQPAISAALSRLRNLFDDPLFVRTGR 61

Query: 3272 RLRPTVRALALMQEVERSFIGLEQIAARASELRTQATGRL-RIACLPALAHALVPRALVY 3096
             + P+ RA  +   +  +   +    +RA+E     +  + RI        AL+P+ L  
Sbjct: 62   SMEPSARAHEIFALLSPALDSISTAVSRAAEFDPATSNAVFRIGLSDDAEFALLPQLLKR 121

Query: 3095 LSHTHPELGVSVHPLESPWLEQAMSEQRFDLGLSETQKAPA 2973
            +    P + + V  +    +   ++     +G+S T + PA
Sbjct: 122  IRAEAPGIVLVVRRVNYLLMPTLLASGEISVGVSYTSELPA 162



>gi|7801208|emb|CAB91105.1| TtgS protein [Pseudomonas putida]
          Length = 304

 Score = 45.8 bits (107), Expect = 9e-04
 Identities = 39/161 (24%), Positives = 68/161 (42%), Gaps = 1/161 (0%)
 Frame = -3

Query: 3452 LFNIDMNLTHRQLTMFRAIMLHGNLGRAADACASSQPTLSRELGRLEQLLGFNLFDRVRG 3273
            L  +D NL    L +F  +M   ++ RAA+     QP +S  L RL  L    LF R   
Sbjct: 6    LRRVDRNL----LIVFETLMHERSVTRAAEKLFLGQPAISAALSRLRNLFDDPLFVRTGR 61

Query: 3272 RLRPTVRALALMQEVERSFIGLEQIAARASELRTQATGRL-RIACLPALAHALVPRALVY 3096
             + P+ RA  +   +  +   +    +RA+E     +  + RI        AL+P+ L  
Sbjct: 62   SMEPSARAHEIFALLSPALDSISTAVSRAAEFDPATSNAVFRIGLSDDAEFALLPQLLKR 121

Query: 3095 LSHTHPELGVSVHPLESPWLEQAMSEQRFDLGLSETQKAPA 2973
            +    P + + V  +    +   ++     +G+S T + PA
Sbjct: 122  IRAEAPGIVLVVRRVNYLLMPTLLASGEISVGVSYTSELPA 162



>gi|13926035|gb|AAK49514.1|AF321092_1 TtgS [Pseudomonas putida]
          Length = 112

 Score = 38.9 bits (89), Expect = 0.11
 Identities = 25/91 (27%), Positives = 43/91 (47%)
 Frame = -3

Query: 3452 LFNIDMNLTHRQLTMFRAIMLHGNLGRAADACASSQPTLSRELGRLEQLLGFNLFDRVRG 3273
            L  +D+NL    L +F  +M   ++ RAA+     QP +S  L RL  L    LF R   
Sbjct: 6    LRRVDLNL----LIVFETLMHERSVTRAAEKLFLGQPAISAALSRLRSLFDDPLFVRTGR 61

Query: 3272 RLRPTVRALALMQEVERSFIGLEQIAARASE 3180
             + P+ RA+ +   +  +   +    +RA++
Sbjct: 62   SMEPSARAVEIFALLSPALDSISTAVSRAAD 92



>gi|149824947|gb|EDM84159.1| acriflavin resistance protein
            [Limnobacter sp. MED105]
          Length = 1025

 Score = 36.6 bits (83), Expect = 0.53
 Identities = 26/87 (29%), Positives = 42/87 (48%), Gaps = 7/87 (8%)
 Frame = +1

Query: 3679 NSNTHILRL----MKRQGVVVDSVSLGEIERALASGFTPGLH---NGHADIVFTADLLDR 3837
            N N  ++++     + + + V S S+ E    + SG T G +   N   DIVF     +R
Sbjct: 711  NENIKVMKVEVDQARARALGVSSQSIAESSHVILSGATIGQYRENNRLIDIVFRNPESER 770

Query: 3838 ATLARVVELNIPVNCGSMDMLDQLGAV 3918
             TLA ++  N+P   G+   L Q+G V
Sbjct: 771  DTLAELMNANVPTASGAYVPLSQVGKV 797



>gi|149926129|ref|ZP_01914391.1| acriflavin resistance protein
            [Limnobacter sp. MED105]
          Length = 1025

 Score = 36.6 bits (83), Expect = 0.53
 Identities = 26/87 (29%), Positives = 42/87 (48%), Gaps = 7/87 (8%)
 Frame = +1

Query: 3679 NSNTHILRL----MKRQGVVVDSVSLGEIERALASGFTPGLH---NGHADIVFTADLLDR 3837
            N N  ++++     + + + V S S+ E    + SG T G +   N   DIVF     +R
Sbjct: 711  NENIKVMKVEVDQARARALGVSSQSIAESSHVILSGATIGQYRENNRLIDIVFRNPESER 770

Query: 3838 ATLARVVELNIPVNCGSMDMLDQLGAV 3918
             TLA ++  N+P   G+   L Q+G V
Sbjct: 771  DTLAELMNANVPTASGAYVPLSQVGKV 797



>gi|126098068|gb|ABN74896.1| probable macrolide-specific efflux
            protein [Actinobacillus pleuropneumoniae L20]
          Length = 258

 Score = 34.7 bits (78), Expect = 2.0
 Identities = 34/136 (25%), Positives = 60/136 (44%), Gaps = 7/136 (5%)
 Frame = -3

Query: 7652 ASTKDQDAKRALQILQDLNQNLNLGETIVYVENYSGTKLDRPELNKLLSE------ANQG 7491
            A ++   AK  LQI Q+     NL    + VE  + T+L   E+   L        A  G
Sbjct: 94   AKSRLNTAKAELQIAQN-----NLDIAKIRVEK-AETELGYTEIRSPLDATVISVFAQNG 147

Query: 7490 DTLLV-ESIDRLSRLTQRDFQELKRRIQEKGLRLVVADLPTTYQMIQASDSITHSILELI 7314
             TL+  + +  L +L   D  ++  +I E  +  + A  PT++ ++  SDS  H  L+++
Sbjct: 148  QTLVTTQQVPVLMKLANIDTMKVNTKISEADVIHIQAGAPTSFTLLGESDSQFHGQLDMV 207

Query: 7313 NNMLIDLLATMARLDN 7266
                + +  T    +N
Sbjct: 208  KLAPVHITDTTENTNN 223



>gi|126209276|ref|YP_001054501.1| probable macrolide-specific efflux
            protein [Actinobacillus pleuropneumoniae L20]
          Length = 258

 Score = 34.7 bits (78), Expect = 2.0
 Identities = 34/136 (25%), Positives = 60/136 (44%), Gaps = 7/136 (5%)
 Frame = -3

Query: 7652 ASTKDQDAKRALQILQDLNQNLNLGETIVYVENYSGTKLDRPELNKLLSE------ANQG 7491
            A ++   AK  LQI Q+     NL    + VE  + T+L   E+   L        A  G
Sbjct: 94   AKSRLNTAKAELQIAQN-----NLDIAKIRVEK-AETELGYTEIRSPLDATVISVFAQNG 147

Query: 7490 DTLLV-ESIDRLSRLTQRDFQELKRRIQEKGLRLVVADLPTTYQMIQASDSITHSILELI 7314
             TL+  + +  L +L   D  ++  +I E  +  + A  PT++ ++  SDS  H  L+++
Sbjct: 148  QTLVTTQQVPVLMKLANIDTMKVNTKISEADVIHIQAGAPTSFTLLGESDSQFHGQLDMV 207

Query: 7313 NNMLIDLLATMARLDN 7266
                + +  T    +N
Sbjct: 208  KLAPVHITDTTENTNN 223



>gi|149822944|gb|EDM82189.1| RND family efflux transporter
            [Limnobacter sp. MED105]
          Length = 840

 Score = 34.7 bits (78), Expect = 2.0
 Identities = 22/67 (32%), Positives = 37/67 (55%), Gaps = 2/67 (2%)
 Frame = -2

Query: 6567 SENGHSESMVELADLYTRADSGIEVNIKKALELREK--AAKLGNKKAMRSLSVMYRDGIG 6394
            S NG   S  +L ++YT +D+G  V +   ++++E+  AA+L      R++ +     IG
Sbjct: 554  SSNGSLNSPFDLLNIYTSSDTGQLVPLASMIKVQERGVAAELDRHAQRRAVEL----NIG 609

Query: 6393 IPKNTDL 6373
            IP  TDL
Sbjct: 610  IPPGTDL 616



>gi|149928359|ref|ZP_01916600.1| RND family efflux transporter
            [Limnobacter sp. MED105]
          Length = 840

 Score = 34.7 bits (78), Expect = 2.0
 Identities = 22/67 (32%), Positives = 37/67 (55%), Gaps = 2/67 (2%)
 Frame = -2

Query: 6567 SENGHSESMVELADLYTRADSGIEVNIKKALELREK--AAKLGNKKAMRSLSVMYRDGIG 6394
            S NG   S  +L ++YT +D+G  V +   ++++E+  AA+L      R++ +     IG
Sbjct: 554  SSNGSLNSPFDLLNIYTSSDTGQLVPLASMIKVQERGVAAELDRHAQRRAVEL----NIG 609

Query: 6393 IPKNTDL 6373
            IP  TDL
Sbjct: 610  IPPGTDL 616



>gi|108736213|gb|ABG00342.1| RepB [Lactococcus lactis]
          Length = 282

 Score = 33.9 bits (76), Expect = 3.4
 Identities = 21/99 (21%), Positives = 46/99 (46%)
 Frame = -2

Query: 9879 NSLILLDSHKRFYVKQLIKKDICISFLETIIFLIIYLIR*GIRKNKVIRMSRDSNAAEAL 9700
            N ++ +D    + V+Q   KD+ +S +E +  L  Y+      K      S +      L
Sbjct: 73   NEIMTIDKTLMWGVQQRDLKDLPVSIIENLDLLPSYIDFEDFAKYLYKNTSNEYEETHLL 132

Query: 9699 ADLLFPQEEDFAENILDIPPVQRKLHTETYDFTVQMIVN 9583
              L  P +ED+   ++D+PP+  ++ +    F+  ++++
Sbjct: 133  EPLFEPLKEDYDIILIDVPPLSVEVTSNAVMFSDYVLIS 171



>gi|91710870|gb|ABE50798.1| acriflavin resistance protein
            [Methylobacillus flagellatus KT]
          Length = 1067

 Score = 33.5 bits (75), Expect = 4.5
 Identities = 17/50 (34%), Positives = 26/50 (52%), Gaps = 3/50 (6%)
 Frame = +1

Query: 3805 DIVFTADLLDRATLARVVELNIPVNCGSMDMLDQLGAVNPGHP---VWLR 3945
            D+V   D L+R  L+ + EL +P + G+   L QL  + PG     +W R
Sbjct: 790  DLVLRGDTLERTHLSHLPELMVPTSTGTSVPLSQLATLTPGFEDAVIWRR 839



>gi|91776883|ref|YP_546639.1| acriflavin resistance protein
            [Methylobacillus flagellatus KT]
          Length = 1067

 Score = 33.5 bits (75), Expect = 4.5
 Identities = 17/50 (34%), Positives = 26/50 (52%), Gaps = 3/50 (6%)
 Frame = +1

Query: 3805 DIVFTADLLDRATLARVVELNIPVNCGSMDMLDQLGAVNPGHP---VWLR 3945
            D+V   D L+R  L+ + EL +P + G+   L QL  + PG     +W R
Sbjct: 790  DLVLRGDTLERTHLSHLPELMVPTSTGTSVPLSQLATLTPGFEDAVIWRR 839




  Database: /dbase/web_blast/db/Efflux_Pump.fasta
    Posted date:  Aug 12, 2007 10:30 PM
  Number of letters in database: 14,206,867
  Number of sequences in database:  24,506
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 269,586,314
Number of Sequences: 24506
Number of extensions: 5272126
Number of successful extensions: 14760
Number of sequences better than 10.0: 13
Number of HSP's better than 10.0 without gapping: 10492
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 14752
length of database: 14,206,867
effective HSP length: 122
effective length of database: 11,217,135
effective search space used: 48749668710
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)